Code | CSB-E09257h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human TARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:5 | Average % | 106 | |||||
Range % | 101-115 | ||||||
1:10 | Average % | 104 | |||||
Range % | 102-108 | ||||||
1:20 | Average % | 87 | |||||
Range % | 84-93 | ||||||
1:40 | Average % | 94 | |||||
Range % | 90-97 |
The recovery of human TARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 96 | 90-101 | |||||
EDTA plasma (n=4) | 89 | 85-92 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human TARC ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human TARC in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the TARC levels in the sample and is measured at 450 nm through a microplate reader.
TARC, also called CCL17, is constitutively expressed in the thymus and produced by dendritic cells (DCs), endothelial cells, keratinocytes, and bronchial epithelial cells. CCL17 plays an important role in T cell development in the thymus, and it binds to CCR4 and displays chemotactic activity for T lymphocytes, predominantly Th2 cells. Moreover, some studies have demonstrated that chemokine CCL17, an important regulator of atherosclerosis, is positively related to coronary artery disease, independent of traditional cardiovascular risk factors. Another study has revealed that circulating CCL17 plays a crucial role in aging as well as Ang II-induced pathological cardiac remodeling and heart failure (HF).
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