Human Pregnancy-specific beta-1-glycoprotein 9(PSG9) ELISA kit

Code CSB-EL018859HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
pregnancy specific beta-1-glycoprotein 9
Alternative Names
pregnancy specific beta 1 glycoprotein (PSG) ELISA Kit; pregnancy specific beta 1 glycoprotein 11 ELISA Kit; pregnancy specific beta 1 glycoprotein 9 ELISA Kit; pregnancy specific beta 1 glycoprotein B ELISA Kit; Pregnancy-specific beta-1 glycoprotein B ELISA Kit; Pregnancy-specific beta-1-glycoprotein 11 ELISA Kit; Pregnancy-specific beta-1-glycoprotein 9 ELISA Kit; Pregnancy-specific glycoprotein 11 ELISA Kit; Pregnancy-specific glycoprotein 7 ELISA Kit; Pregnancy-specific glycoprotein 9 ELISA Kit; PS beta B ELISA Kit; PS-beta-B ELISA Kit; PS-beta-G-11 ELISA Kit; PS-beta-G-9 ELISA Kit; PS34 ELISA Kit; PSBG 9 ELISA Kit; PSBG-11 ELISA Kit; PSBG-9 ELISA Kit; PSG 9 ELISA Kit; PSG11 ELISA Kit; PSG7 ELISA Kit; PSG9 ELISA Kit; PSG9_HUMAN ELISA Kit; PSGII ELISA Kit
Abbreviation
PSG9
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
5 μg/mL-150 μg/mL
Sensitivity
1.25 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Tags & Cell Markers
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PSG9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 95    
Range % 91-99    
1:2 Average % 89    
Range % 83-99    
1:4 Average % 98    
Range % 90-105    
1:8 Average % 92    
Range % 88-100    
Recovery
The recovery of human PSG9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 93-100    
EDTA plasma (n=4) 96 91-99    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
μg/ml OD1 OD2 Average Corrected    
150 2.523 2.514 2.519 2.471    
62.5 1.308 1.317 1.313 1.265    
25 0.624 0.609 0.617 0.569    
10 0.316 0.305 0.311 0.263    
5 0.156 0.152 0.154 0.106    
0 0.047 0.048 0.048      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PSG9 ELISA Kit was designed for the quantitative measurement of Human PSG9 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 5 μg/mL-150 μg/mL and the sensitivity is 1.25 μg/mL.

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Target Background

Gene References into Functions
  1. Results indicate that pregnancy-specific glycoprotein 9 (PSG9) may facilitate the development of hepatocellular carcinoma (HCC) by fostering angiogenesis via promoting VEGFA production in cancer HCC. PMID: 28078509
  2. Findings illustrate the innovative mechanism by which PSG9 drives the progression of colorectal cancer and tumor angiogenesis. This occurs via nuclear translocation of PSG9/SMAD4, which activates angiogenic cytokines. PMID: 27528036
  3. PSG9, through its activation of TGFbeta-1, could be a potent inducer of immune tolerance PMID: 27389696
  4. PSG9 may have a role in development of colorectal carcinogenesis in patients with adenomatous polyposis coli PMID: 15982419
Subcellular Location
Secreted.
Protein Families
Immunoglobulin superfamily, CEA family
Database Links

HGNC: 9526

OMIM: 176398

KEGG: hsa:5678

STRING: 9606.ENSP00000270077

UniGene: Hs.502092

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