Code | CSB-MP019483HU |
MSDS | |
Size | $228 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Human retinol-binding protein 4 (RBP4) amino acid Glu19-Leu201 linked a C-terminal human IgG1 Fc tag by the TEV linker, as well as an N-terminal linker, was expressed in mammalian cells. The product is the C-terminally hFc-tagged recombinant human RBP4 protein. This recombinant RBP4 protein is an active protein, whose bioactivity has been measured by its binding with the TTR in a functional ELISA, with the EC50 of 695.0-970.1 ng/ml. The purity of this protein is greater than 90% determined by SDS-PAGE. It has an apparent molecular weight of 55 kDa on the gel while its predicted mass is 50 kDa. It contains less endotoxin (<1.0 EU/ug protein), measured by the LAL method. Its protein length covers the full length of the mature RBP4 protein. It is in stock now.
RBP4, mainly synthesized in the liver and adipose tissue, facilitates the transport of retinol from the liver to the peripheral organs. It is also an adipokine and fatty acid transporter. Besides, RBP4 is one of the adipokines involved in the development of insulin resistance.
Applications : Binding assay/Protein-protein interaction
Review: The ELISA verification showed that RBP4 has good binding activity with TTR and can be used for subsequent method development. The protein has good affinity, is relatively stable, has detailed instructions, is easy to dissolve, and with no significant difference between different batches, making it very useful. This experiment has progressed smoothly and has greatly helped us in our research!
By Anonymous