Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized RBP4 (CSB-MP019483HU) at 5 μg/ml can bind human TTR, the EC50 of human TTR protein is 284.7-391.7 ng/ml.
Alternative Names
ATTR (Prealbumin) (TBPA) (PALB)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
21-147aa
Target Protein Sequence
GPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal FC-Myc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Human transthyretin (TTR) amino acids Gly21-Glu147 with an FC-Myc-tag at the C-terminus were expressed in mammalian cells. The resulting product is the recombinant full-length of mature human TTR protein. This TTR protein was validated by SDS-PAGE analysis, and its purity is greater than 90%. It migrated to the molecular mass band of about 45 kDa on the gel. Its endotoxin level is less than 1.0 EU/ug as determined by the LAL method. This TTR protein can bind to the immobilized RNP4 in the functional ELISA, with an EC50 of 284.7-391.7 ng/ml. It shows the TTR is an active protein. And it is available now.
TTR is a tetrameric transport protein mainly synthesized in the liver, choroid plexus, and retinal pigment epithelium. In addition to transporting thyroxin and retinol throughout the body and brain, TTR has recently been shown to be involved in neuronal health preservation in both central and peripheral nervous systems, cellular fate determination, as well as modulation of proliferation and metabolism.