The outbreak of SARS-CoV-2 has become a global pandemic and an unprecedented public health issue. There are several methods to detect the presence of SARS-CoV-2 in samples, including RT-qPCR, immunochromatography (rapid test) and ELISA. Current testing for the SARS-CoV-2 virus is limited, and compared to RT-qPCR, ELISA is a less complex procedure that uses more affordable and available equipment. The ELISA technique, unlike the other two methods, is currently more intended for research of COVID-19. This technique makes it possible to measure either SARS-CoV-2 antigens or antibodies to these antigens. CUSABIO has developed 3 types of ELISA kits to help you in your research.
N protein encoded by the ORF9b gene is the most abundant protein in coronaviruses. This protein is a highly immunogenic phosphoprotein involved in viral genome replication and modulation of cell signalling pathways. S protein, a type I transmembrane protein, is present on SARS-CoV-2 surface in the form of a homotrimer. Each monomer consists of 2 subunits, S1 and S2. This protein plays an important role in the infection of SARS-CoV-2 as well as in the induction of neutralizing antibody. CUSABIO antigen detection ELISA kit aims to quantitatively determine the concentration of SARS-CoV-2 antigen protein in serum, plasma and swabs.
Name | Code | Detected Antigen | Sample Types | Detection Range | Sensitivity | Assay Principle | Measurement |
---|---|---|---|---|---|---|---|
SARS-CoV-2 N ELISA Kit | CSB-EL33251 | N | serum, plasma, swabs | 3.12 ng/mL-200 ng/mL | 0.78ng/mL | Quantitative | Sandwich |
SARS-CoV-2 S1 RBD ELISA Kit | CSB-EL33244 | S1 RBD | serum, plasma, swabs | 1.25-80ng/mL | 0.31ng/mL | Quantitative | Sandwich |
* For research use only
* 24T ELISA Kit Trial Size is Available. Please click the product name to apply now!
Principle of the assay: These two ELISA kits are based on double antibody sandwich method. Taking the SARS-CoV-2 N ELISA kit as an example (Figure 1), antibody (also called capture antibody) specific for SARS-CoV-2 N has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SARS-CoV-2 N present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SARS-CoV-2 N binds to the antigen. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then the enzyme reacts with its substrate to produce a visible signal that can be measured.
CUSABIO provides four antibody detection ELISA assays for SARS-CoV-2/ COVID19 Research. The antibodies involved in these assays include IgG and IgM. IgG is the most abundant antibody isotype (70~75%) in the blood. Moreover, infected patients develop IgG against SARS-CoV-2 S1 RBD or N 7 days post-infection, and IgG content peaks in the third week. IgG is predominantly responsible for long term immunity after infection. IgM accounts for ~10% of antibodies in the blood and is produced first in response to acute infection.
Name | Code | Detected Antibody | Antigen | Sample Types | Assay Principle | Measurement |
---|---|---|---|---|---|---|
Human SARS-CoV-2 N IgG Antibody ELISA Kit | CSB-EL3325HU | N IgG | N | serum, plasma | Qualitative | Indirect |
Human SARS-CoV-2 N/S1 IgG Antibody ELISA Kit | CSB-EL3326HU | N/S1 IgG | N/S1 | serum, plasma | Qualitative | Indirect |
Human SARS-CoV-2 S1 RBD IgG Antibody ELISA Kit | CSB-EL33241HU | S1 RBD IgG | S1 RBD | serum, plasma | Qualitative | Indirect |
Human SARS-CoV-2 S1 RBD IgM Antibody ELISA Kit | CSB-EL33242HU | S1 RBD IgM | S1 RBD | serum, plasma | Qualitative | Indirect |
* For research use only
* 24T ELISA Kit Trial Size is Available. Please click the product name to apply now!
Principle of the assay: The four ELISA assays are based on indirect method. Taking SARS-CoV-2 S1 RBD IgG Antibody ELISA Kit as an example (Figure 2), the microtiter plate provided in this kit has been pre-coated with human antigen SARS-CoV-2 S1 RBD. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human SARS-CoV-2 S1 RBD IgG antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
In addition to the above two types of SARS-CoV-2 assays, CUSABIO has also developed SARS-CoV-2 neutralizing antibody ELISA kit for the qualitative determination of SARS-CoV-2 neutralizing antibody in serum, plasma.
Name | Code | Detected Antibody | Antigen | Sample Types | Assay Principle | Measurement |
---|---|---|---|---|---|---|
SARS-CoV-2 Neutralizing Antibody ELISA Kit | CSB-EL33243HU | Neutralizing Antibody | S1 RBD | serum, plasma | Qualitative | Competitive |
* For research use only
* 24T ELISA Kit Trial Size is Available. Please click the product name to apply now!
Principle of the assay: This assay is based on competitive method (Figure 3). The microtiter plate provided in this kit has been pre-coated with RBD. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated ACE2. The competitive inhibition reaction is launched between with HRP-ACE2 and SARS-CoV-2 neutralizing antibody in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of SARS-CoV-2 neutralizing antibody in the sample. The color development is stopped and the intensity of the color is measured.
SARS-CoV-2 Antibodies & Proteins
SARS-CoV-2 ELISA Kit